Characterization of Genetic Miscoding Lesions Caused by Postmortem Damage

Recharacterization of ancient DNA miscoding lesions: insights in the era of sequencing-by-synthesis

Although ancient DNA (aDNA) miscoding lesions have been studied since the earliest days of the field, their nature remains a source of debate. A variety of conflicting hypotheses exist about which miscoding lesions constitute true aDNA damage as opposed to PCR polymerase amplification error. Furthermore, considerable disagreement and speculation exists on which specific damage events underlie o...

Statistical evidence for miscoding lesions in ancient DNA templates.

It is generally believed that sequence heterogeneity in PCR products from fossil remains are due to regular DNA polymerase errors as well as miscoding lesions compounded by damage in the template DNA (Pääbo 1990; Handt et al. 1994b, 1996; Höss et al. 1996; Krings et al. 1997). However, it has been difficult to test the frequency with which this assumption holds. First, DNA extractions from foss...

Analysis of the theory of thermal damage in biological tissues caused by Laser beam

Introduction: In laser surgery, the laser beam can evaporate and cut the tissue like a small surgical knife when the tissue temperature is heated to 100 degrees Celsius. a complete understanding of the distribution of damage in both pathologic tissue and surrounding tissue is necessary. Although the test is the most realistic solution for treating medical problems, for this rea...

Ocular damage caused by Paraquat.

Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein

The lack of a mutant phenotype in homozygous mutant individuals' due to compensatory gene expression triggered upstream of protein function has been identified as genetic compensation. Whilst this intriguing process has been recognized in zebrafish, the presence of homozygous loss of function mutations in healthy human individuals suggests that compensation may not be restricted to this model. ...